14-3-3η is a novel growth-promoting and angiogenic factor in hepatocellular carcinoma

Jian Shen, Fei Jiang, Ye Yang, Guangming Huang, Fuxing Pu, Qinqiang Liu,Lijun Chen, Liang Ju, Ming Lu, Fei Zhou, Chi Zhang, Xiagang Luo,XiaojunYang, Chengyu Jiao, Xiangcheng Li, Zhong Li, Yuan Li, Jianping Zhang

Journal of Hepatology November 2016Volume 65, Issue 5, Pages 953–962 DOI: http://dx.doi.org/10.1016/j.jhep.2016.05.017

……For cell transfection, the pcDNA-3.1-14-3-3-Flag plasmid that overexpressed both 14-3-3 and Flag was created by inserting the coding sequences of 14-3-3 (YWHAH, 741 bp) into pcDNA3.1 plasmid, followed by adding a Flag tag at its N-terminal (Generay Biotech Co. Ltd, Shanghai, China).……