Primer Synthesis Services
GENERAY Biotech Primer Synthesis Department has a GMP-grade primer synthesis laboratory and an industry-standard primer synthesis production line to provide high-quality products for scientific research users and the molecular diagnostics (IVD) industry. The company's leading products are Taqman probes, molecular beacons, double quenching probes, LNA probes, capture probes, DEL primers, NGS primers, RAA probes, RNA synthesis and other customized synthesis. The single synthesis specifications range from 2 OD to 10000 OD, and the synthesis length reaches 150base.
GENERAY Biological has five synthetic bases in Shanghai, Beijing, Guangzhou, Nanjing, and Hangzhou, providing faster and more considerate services for various users!
Common Primer:15-60bp
Low mutation rate: less than 1/1000
Provide 1~10 000OD specifications (can provide users with 96-well plate or 384-well plate package delivery)
Provide multiple purification methods including DSL, F-PAGE, HPLC and PAGE
Long-chain Primer: 61-90bp
Purity of polypropylene gel electrophoresis according to international standards
High-standard long-chain primers suitable for experimental requirements such as NGS
Modified / Labeled (Probe) Services
GENERAY Biotech Primer Synthesis provides multiple types of modified / labeled primers, including: fluorescent probe synthesis services, capture probe synthesis services, fluorescent in situ hybridization services, qPCR probe design and synthesis services. Available modification types are: FAM, HEX, TET, TAMRA, ROX, VIC, NED, Alexa Flour, ATTO series, CY series, etc., amino modification, thiol modification, biotin, electrochemical labeling, Fluorescence, BHQ0, BHQ1, BHQ2, BHQ3, Dabcyl, ECL, NFQ, FAM-dT, BHQ-dT, dSpacer et.al.
Base Modification
|
Modification |
Purification way |
|
Phosphorothioate (S-oligos)* |
PAGE/HPLC |
|
Deoxylnosine (dI) |
PAGE/HPLC |
|
DeoxyUridine (dU) |
PAGE/HPLC |
|
5-Methyl dC |
PAGE/HPLC |
|
2'-OMe- C |
PAGE/HPLC |
|
2'-OMe- G |
PAGE/HPLC |
|
2'-OMe- T |
PAGE/HPLC |
|
2'-OMe- A |
PAGE/HPLC |
|
5-Carboxy-Dc (5Cac) |
PAGE/HPLC |
|
5-Hydroxymethyl-dC (5hmc) |
PAGE/HPLC |
|
5-Formyl-dC (5fmc) |
PAGE/HPLC |
|
N6-Me-dA |
PAGE/HPLC |
|
2’-F-base |
PAGE/HPLC |
|
LNA-dA/dG/dC/dT |
PAGE/HPLC |
|
Note: HPLC purification method is charged separately |
|
Single Labeled Probe- Fluorescent Modification
|
Modification |
5’ modified |
Middle modified |
3’ modified |
|
FAM |
√ |
√ |
√ |
|
FITC |
√ |
√ |
√ |
|
HEX |
√ |
√ |
√ |
|
SIMA |
√ |
× |
√ |
|
JOE |
√ |
√ |
√ |
|
ROX |
√ |
√ |
√ |
|
Rhodamine Green |
√ |
√ |
√ |
|
Rhodamine Red |
√ |
√ |
√ |
|
TAMRA |
√ |
√ |
√ |
|
TET |
√ |
√ |
√ |
|
Texas Red |
√ |
√ |
√ |
|
Cy3 |
√ |
√ |
√ |
|
Cy5 |
√ |
√ |
√ |
|
AMCA |
√ |
√ |
√ |
|
BHQ -1 |
× |
√ |
√ |
|
BHQ -2 |
× |
√ |
√ |
|
Eclipse |
× |
× |
√ |
|
Dabsyl |
× |
√ |
√ |
|
Fluorescein |
√ |
√ |
√ |
|
ATTO550 |
√ |
√ |
√ |
|
ATTO565 |
√ |
√ |
√ |
|
ATTO633 |
√ |
√ |
√ |
|
Yakima Yellow |
√ |
× |
√ |
|
iFluor™ 488 |
√ |
√ |
√ |
|
iFluor™ 532 |
√ |
√ |
√ |
|
iFluor™ 555 |
√ |
√ |
√ |
|
iFluor™ 648 |
√ |
√ |
√ |
|
iFluor™ 750 |
√ |
√ |
√ |
Double Probe Modification
|
5’-Fluorophore |
3'-Quencher |
|
5'- FAM |
3'- TAMRA |
|
3'- Dabcyl |
|
|
3'- BHQ1 |
|
|
3'-Eclipse |
|
|
3'-MGB |
|
|
5'- HEX |
3' TAMRA |
|
3'- Dabcyl |
|
|
3'- BHQ1 |
|
|
3'- BHQ2 |
|
|
3'-Eclipse |
|
|
3'-MGB |
|
|
5'-TET |
3'- Dabcyl |
|
3'- TAMRA |
|
|
3'- BHQ1 |
|
|
3'- BHQ2 |
|
|
3'-Eclipse |
|
|
5'-JOE |
3'- TAMRA |
|
3'- Dabcyl |
|
|
3'- BHQ2 |
|
|
5'-ROX |
3'- BHQ2 |
|
3'-Eclipse |
|
|
3'-MGB |
|
|
5'-Cy3 |
3'- Dabcyl |
|
3'-Eclipse |
|
|
3'- BHQ2 |
|
|
5'-Cy5 |
3'- Dabcyl |
|
3'-Eclipse |
|
|
3'- BHQ3 |
|
|
3'-MGB |
|
|
5'- TAMRA |
3'- BHQ2 |
|
3'-Eclipse |
|
|
5’-VIC |
3'-MGB |
|
5’-NED |
3'-MGB |
Chemical Modification
|
Modification |
5’ modified |
Middle modified |
3’ modified |
|
Phosphorylation |
√ |
√ |
√ |
|
Aminolinker |
√ |
√ |
√ |
|
Spacers |
√ |
√ |
√ |
|
Thiolation |
√ |
√ |
√ |
|
Biotin |
√ |
√ |
√ |
|
Biotin TEG |
× |
√ |
√ |
|
Digoxigenin |
√ |
√ |
√ |
Connection Probe
|
Modification |
Purification way |
|
Triple SH |
HPLC |
|
Maleimide |
HPLC |
|
Azide(N3) |
HPLC |
|
CHCH |
HPLC |
|
DBCO |
HPLC |
|
COOH |
HPLC |
|
Acryl |
HPLC |
Please call for other Modifications
|
More Modification |
|||
|
VIC |
Alexa fluor 488 |
N6-Me-dA |
AMCA |
|
MAX |
Alexa Fluor 532 |
Azobenzene |
Acrydite |
|
NED |
Alexa fluor 546 |
Bromo dU |
CY3.5 |
|
PET |
Alexa fluor 555 |
1-Me-dA |
CY5.5 |
|
TAZ |
Alexa fluor 568 |
PC-Linker |
CY7.5 |
|
SID |
Alexa fluor 633 |
Inverted base |
QSY |
|
LIZ |
Alexa fluor 647 |
Pyrene |
APhluor 593 |
|
Qusar570 |
Alexa fluor 750 |
Symmetric |
IRDye ® |
|
Qusar670 |
Bodipy 530/550 |
CHO |
5' Terminal Caps |
Commonly used Fluorescent Dye Parameters
|
Dye |
Excitation(nm) |
Emission(nm) |
Colour |
|
6-FAM |
494 |
518 |
Yellow-Green |
|
Flurescein (FITC) |
495 |
520 |
|
|
TET |
521 |
536 |
|
|
JOE |
520 |
548 |
Yellow |
|
HEX |
533 |
558 |
|
|
CY3 |
550 |
570 |
Yellow-Orange |
|
TAMRA |
544 |
576 |
|
|
ROX |
576 |
601 |
Orange |
|
CY5 |
650 |
670 |
Red |
Synthesis Features
Advanced synthesis equipment: high-throughput Dr.Oligo series and large-scale Mermade series synthesizers
Various purification methods: DSL, F-PAGE, HPLC and PAGE
Good product quality: relying on ESI-MS mass spectrometer and HPLC chromatography for quality control
Fast synthesis speed: Set up synthesis bases in Shanghai, Beijing, Guangzhou (Kunming), Nanjing, and Hangzhou to fully guarantee the synthesis speed
Product Quality
Correctness: Strict quality control standards and QC methods ensure product correctness
Accuracy: Retest the concentration of each probe and compare with the standard, accurately control the difference between batches, and ensure the accuracy of the product
Stability: using unique technology to reduce the damage of fluorescein during purification
Quality control: perfect central control, real-time monitoring of the coupling efficiency of each base, to ensure that the efficiency of each step is greater than 99%; ESI-MS mass spectrometry, HPLC, full-wavelength scanning, and fluorescence spectrum detection PAGE analysis gel detection and CE capillary electrophoresis detection 6-fold QC
Primer Purification Guide
|
Primer |
Purification Way |
|||
|
DSL |
F-PAGE |
PAGE |
HPLC |
|
|
＜15nt |
Applicable |
Not Applicable |
Not Applicable |
Recommend |
|
15-40nt |
Recommend |
Recommend |
Applicable |
Recommend |
|
41-59nt |
Applicable |
Applicable |
Recommend |
Applicable |
|
60-120nt |
Not Applicable |
Not Applicable |
Recommend |
Applicable（60-100nt） |
|
Scope of application |
PCR amplification, whole gene synthesis, DNA sequencing |
DNA sequencing, whole gene synthesis, site-directed mutation, PCR cloning, quantitative PCR, multiplex PCR, point mutation / subcloning, RNA interference / gene construction |
Purity requirements for next-generation sequencing, diagnostic PCR, mutation library construction, and protein-binding gel migration electrophoresis analysis |
Dot cloning, subcloning, diagnostic PCR and protein binding gel migration electrophoresis analysis; modified primers with hydrophobic groups; commercial diagnostic primers and probes |
DNA Production Quality Control Flow Chart
DNA Production Quality Control Report-HPLC Primer purity test
Explanation of test results: In the figure, the area of the peak accounts for 100% . So the test is qualified.
Eligibility criteria: The main peak area is 90% or more.
DNA Production Quality Control Report-MS
MS test result description: Observing the theoretical molecular weight and actual molecular weight, it can be found that the theoretical molecular weight of this primer are 7464.06、5537.86、9357.35、 7820.35 and 8190.07, and the actual molecular weight are 7465.0、5538.7、9358.6、7822.4 and 8187.2. The test is qualified.
Eligibility criteria: The measured molecular weight and the molecular weight less than 0.1% difference theory
Have a Question?
Order Online: dna@generay.com.cn
By Phone: 86-21-67840801/67840802
