RNA Synthesis Services
As an important research tool, RNA oligo is widely used in research applications such as gene function analysis and development of novel therapeutic strategies. GENERAY Biotechnology provides high-quality customized services for RNA synthesis, which can meet the different needs of our customers. RNA synthesis services include: General RNA synthesis, RNA modification and labeling, Chimeric RNA, 2'-OMe-RNA and other antisense RNAs. In order to ensure the high quality of RNA oligo, synthetic RNA strictly implements QC testing standards. Through MS, HPLC and other detection methods to ensure that the final product delivered to customers is high quality RNA oligo.
1.siRNA and Single RNA
siRNA synthesis is the easiest way to achieve gene silencing in the body or cells, with low cost and high transfection efficiency. It is also the best method for clinical treatment of RNAi. It has irreplaceable advantages in drug development. GENERAY Biotechnology can provide customers with common chemically synthesized siRNAs, and can provide a variety of modified siRNAs according to customer needs. Ordinary chemically synthesized siRNA products are purified by HPLC to completely remove unpaired single strands. Customers only need to dilute with the buffer in the box to perform subsequent
1.1 Regular siRNA synthesis pricelist (15-25bp) double-stranded
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Project description |
Specifications | Purification Way |
Delivery Time |
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Conventional siRNA
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2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
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| Note: Conventional siRNA provides a annealed double strand by default, with two overhangs dT at the 3' end | |||
1.2 Modified siRNA synthesis pricelist (15-25bp) double-stranded
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Project description |
Specifications |
Purification Way |
Delivery Time |
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5‘FAM/CY3/CY5
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2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
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5'Biotin/Amino/Phosphate |
2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
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5' Thiol/Cholesterol |
2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
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agomir/ antagomir
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2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
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5’FAM 3'BHQ1 |
2 OD |
HPLC |
5-6 working days |
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5 OD |
HPLC |
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10 OD |
HPLC |
2. siRNA Package
2.1 Regular siRNA Package
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Project description |
specifications |
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siRNA oligo for the target gene |
3 sites |
2 OD |
HPLC |
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Negative control siRNA oligo |
1 pair |
1 OD |
HPLC |
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FAM labeled negative control siRNA oligo |
1 pair |
1 OD |
HPLC |
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RNAi positive control siRNA oligo |
1 pair |
1 OD |
HPLC |
2.2 Modified siRNA Package
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Project description |
specifications |
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siRNA oligo for the target gene |
3 sites |
2 OD |
HPLC |
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Negative control siRNA oligo |
1 pair |
1 OD |
HPLC |
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FAM labeled negative control siRNA oligo |
1 pair |
1 OD |
HPLC |
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RNAi positive control siRNA oligo |
1 pair |
1 OD |
HPLC |
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Note: Full chain methoxy modification, terminal cholesterol modification |
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Package description: To ensure that the transfection efficiency is above 80%, the silence efficiency can reach 70%. Otherwise, design and synthesize again for free.
3、miRNA
GENERAY miRNA products mainly include miRNA Mimic(double-stranded annealing products), miRNA Mimic negative control, miRNA inhibitor (RNA single chain) and miRNA inhibitor negative control products。The miRNA products provided by our company are produced under the quality standard of ISO9001:2015 and have passed repeated optimization and rigorous testing, the product quality is highly stable.
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Project description |
Purification Way |
Cycle |
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miRNA Mimics (Double chain annealing product) |
HPLC |
5-7 working days |
| miRNA Mimics negative control |
HPLC |
5-7 working days |
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miRNA inhibitor (RNA strands) |
HPLC |
5-7 working days |
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miRNA inhibitor negative control |
HPLC |
5-7 working days |
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Note: (1) All products were purified by HPLC and passed strict quality control |
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(2) All RNA is produced in the clean workshop to strictly control endotoxin and meet the requirements of cell level and animal level |
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(3) Provide mass production |
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(4) Partial modified and long-stranded RNAs are not listed and shall be inquire |
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4、shRNA
The shRNA expression vector is labeled with antibiotics and can continuously suppress the expression of target genes in cells for weeks or longer. For a known effective siRNA sequence (for example, obtained by screening by siRNA synthesis method), when it is necessary to maintain gene silencing for a long time, the shRNA vector system is recommended, GENERAY Biological is committed to providing the most advanced and convenient shRNA-related tools, and recently launched a new generation of shRNA expression plasmids, an efficient and ready-to-use vector that can continuously generate shRNAs in cells, thereby achieving a long-lasting inhibition of target gene expression. purpose.
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Project description |
Specifications |
Cycle |
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ShRNA Plasmid expression vector (pGPU6) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPH1) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPU6/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPH1/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPU6/Hygro) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPH1/Hygro) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPU6/GFP/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPH1/GFP/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPU6/RFP/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (pGPH1/RFP/Neo) |
50μg |
2 weeks |
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ShRNA Plasmid expression vector (Plko.1-puro) |
50μg |
2 weeks |
5、Oligo and other coupling
GENERAY Biological can provide PEG, Peptide, chelating agent (NOTA, DOTA, MAG, DTPA) and other small molecule conjugation services with Oligo.
Quality Assurance
Perfect order service system
All RNA oligonucleotides were produced under strict SOP & Quality Control
High resolution Waters chromatography with ESI-MS ensure the purity
Fully traceable ISO 9001 compliance documentation system
Result of Delivery
Synthetic RNA lyophilized powder
COA file
Examination Report (MS & HPLC)
DNA Production Quality Control Flow Chart
RNA Production Quality Control Report-HPLC Primer purity test
Explanation of test results: In the figure, the area of the peak accounts for 100% . So the test is qualified.
Eligibility criteria: The main peak area is 90% or more.
RNA Production Quality Control Report-MS
MS test result description: Since RNA is double-stranded, we provide the theoretical molecular weight and actual molecular weight of the two strands. The test results are shown in the attachment. For example, the first double-stranded RNA test report has theoretical molecular weights of 6725.11 and 6622.08, and actual molecular weights of 6724.7 and 6621.7. So the test is qualified.
Eligibility criteria: The measured molecular weight and the molecular weight less than 0.1% difference theory.
Have a Question?
Order Online：rna@generay.com.cn
By Phone: 021-67840801/67840802
